Telomere is a special structure at the end of a chromosome in a eukaryotic cell. It consists of a DNA with a specific repeat sequence and a telomere-binding protein, which is an important factor in maintaining the stability of the chromosome structure. When the cells become cancerous, due to the reactivation of telomerase, the telomere DNA is gradually degraded with the mitotic activity, and the normal cells are transformed into immortalized malignant cells with infinite ability to divide.
TRF1-interacting ankyrin related ADP-ribose polymerase 1, Tankyrase 1 (also known as PARP5a) is a poly ADP-ribose polymerase associated with telomere function (poly (ADP-ribose) ) polymerase, PARP5a), involved in various regulatory pathways in the body, including Wnt signaling pathways, telomere length regulation, and spindle assembly. Studies have found that changes in telomere function are associated with a variety of physiological changes and disease development, such as cancer. And aging. The Wnt signaling pathway is composed of a series of oncogenes and proteins encoded by tumor suppressor genes, which on the one hand induce the normal development of the organism and on the other hand induce the tumor formation of adult animals. Tankyrase 1 can pass Axis developmental inhibitory factor Axin interacts to promote the degradation of Axin via the ubiquitin-proteasome pathway and activates the Wnt signaling pathway, which in turn affects the transformation and production of cancer cells. Currently, Tankyrase 1 is considered to be a cell carcinogenesis mechanism and cancer. New hotspots in therapeutic target research have attracted the attention of domestic and foreign cancer researchers.
As a leader in the study of tumor cell function and behavior, Trevigen offers a variety of Tankyrase 1 related assay kits including colorimetry and chemiluminescence, which are ideal for screening Tankyrase 1 (PAPR5) inhibitors and determining IC50. . Trevigen's Tankyrase 1 (PAPR5) Colorimetric Activity Assay Kit and Chemiluminescence Activity Assay Kit are highly sensitive screening kits for rapid screening of tankyrase 1 inhibitors in in vitro systems. An ELISA performed on a 96-well plate was able to semi-quantitatively detect poly(ADP-ribose) (PAR) deposited on immunized histones. Anti-PAR monoclonal antibodies, HRP-labeled goat anti-mouse IgG secondary antibodies, and HRP substrates are used to generate detection signals. The activity of Tankyrase 1 can be detected by measuring the OD value at a wavelength of 450 nm by a microplate reader or detecting the luminescence intensity by a chemiluminometer. The kit is easy to operate and features: 1. It is detected by colorimetry and is not radioactive. 2.96 well plate. 3. Ability to detect Tankyrase 1 as low as 0.1 mU units.
Trevigen is a fast-growing US biotechnology company focused on oncology research products and services for apoptosis, DNA damage and repair, tumor cell function and behavior. As Trevigen's general agent in China, Amy Jie and Trevigen provide Chinese researchers with the best and most up-to-date quality products and technical services in areas such as oxidative stress, cell damage and tumor cell behavior research. Article source: http://
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