The early removal of contaminated bacterial cultures into the fermentation room within 3 days is critical. If mold spots appear on the surface of an individual bag, it typically indicates a minor contamination that does not involve spore formation. Under strict aseptic conditions, the left hand should hold the bag upside down while the right hand uses a sterilized resection tool—a thin iron tube about 1 to 1.5 cm in diameter and 25 cm long. Before use, the tool must be flame-sterilized. The contaminated area is carefully cut out using the tube, allowing the mold to be removed through the opening. Afterward, the bag is sealed and returned to cultivation. This method can achieve a success rate of around 90%.
**Re-sterilization and Reuse**
1. Within the first 5 days after entering the fermentation room, if more than 70% of the bags show no germination of seed blocks and no bacterial contamination, the sterilization time was short. In such cases, the entire batch should be immediately re-sterilized and reused.
2. If mold contamination exceeds one-third of the bags within 7 days of entering the fermentation room, regardless of the cause, all contaminated bags must be removed and re-sterilized.
3. After 10 days in the fermentation room, if the mycelium grows slowly or stops, and the raw materials are not at fault, the issue is likely due to insufficient oxygen. In this case, remove the remaining culture, add fresh material, reuse the mixed bacteria, and improve the sealing to ensure better growth.
For late-stage germs, if serious contamination occurs, but more than two-fifths of the bag still contains healthy bacteria or the good strain has taken dominance, the bag can be selected for further use as an inoculum. Experience has shown that under these conditions, the cost can be fully recovered.
When changing mushroom species, small amounts of contamination that cannot be addressed immediately can be stored at low temperatures. Once the black fungus covers the contaminated bag, a small amount of fresh material can be added, and the bag can be re-sterilized to switch the mushroom species. This approach not only reduces the pollution rate but also allows for a 7-day advantage over starting a new batch from scratch.
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