The GST tag system has the advantages of high protein expression yield, convenient purification of expression products, and favorable GST antibody preparation. The GST fusion protein is soluble in aqueous solution, can be extracted from bacterial lysate, obtained by affinity chromatography under invariant conditions, or directly removed by site-specific protease cleavage. Because of the above advantages, commercial GST fusion protein expression systems and GST-tagged antibody systems are still widely used today. When using GST fusion expression system to express foreign genes, it is necessary to detect and purify the products expressed by fusion. This includes the application of GST-tagged antibodies. It is especially important to choose a most cost-effective GST antibody.
How to choose the right GST antibody?
When choosing GST-labeled antibodies, we mainly rely on our own needs, especially the needs of experimental applications. Among the factors to be considered are the type of GST antibody application, the clonality of the GST antibody, and the source of preparation. For example, can the GST antibody be used for Western Bloting? Can it be used for immunoprecipitation IP experiments? Do I need a monoclonal GST-tagged antibody with better specificity? Do I need to consider whether to choose the source of the mouse or the source of the rabbit?
Abbkine's GST antibody has a high titer. Abbkine's HRP-labeled secondary antibody and high-sensitivity luminescence solution were used in our laboratory. Initially, the recommended 1:1000 dilution was used for the WB experiment, and the band was found to be very thick. In the end we used a dilution ratio of 1:10,000, which is ideal, which exceeded our expectations. ——A customer of Peking University Cancer Hospital
| Product name and description | Brand | Item number | specification | product manual |
| Anti-GST Tag Monoclonal Antibody (2A8) | Abbkine | A02030 | 100ul/500ul | Details |
In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies, while polyclonal antibodies Then it has an advantage in affinity. In addition, the titer of the antibody is also an important aspect of investigating the cost performance of the antibody, that is, equivalent to the dilution ratio in practical use. A high titer of 100 μl of GST antibody (if the dilution ratio of WB is 1:5,000, the final use is equivalent to 500 ml), 1 ml of GST antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 2ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.
Why recommend Abbkine brand GST antibodies?
There are many imported brands that supply GST antibodies, such as Abcam, Santa Cruz, Cell Signaling (CST), Millipore, etc., which are related to GST antibodies. However, because it is not a specialized label and supplier of internal reference antibodies, it is not the best choice for cost performance. For example, Abcam, the price of a 100μl GST antibody is ¥4418; the price of a 100μl GST antibody from CST is ¥2997.
Some domestic suppliers can also provide sub-packaged GST antibodies, but there are deficiencies in brand and quality assurance; in general, their potency is not very high. There are also some restrictions on brand after-sales. Here, we recommend the GST-tag antibody from Abbkine, USA - Anti-GST Tag Monoclonal Antibody 
(2A8) (Cat. #A02030) is a monoclonal antibody derived from mouse, clone number is
2A8, the immunogen is a KLP-coupled GST polypeptide.
Abbkine's GST-tagged antibody (Cat. #A02030) is 65% lower than Abcam and only half the price of CST. A high titer antibody not only affects the sensitivity of the experimental results, but also means a higher price/performance ratio. Abbkine's GST-tagged antibody (Cat. #A02030) In the conventional WB experiment, the recommended initial dilution ratio is 1:1000. Actually, in Abbkine applications, there is a dilution ratio of 1:5,000 or higher, depending on the customer. Specific experimental conditions are optimized.
| Product name and description | Brand | Item number | specification | product manual |
| Anti-GST Tag Monoclonal Antibody (2A8) | Abbkine | A02030 | 100ul/500ul | Details |
Abbkine also offers a large package of 1ml GST-tagged antibody, which is ideal for routine testing of GST immunoassays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China , and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to 801/802/803 or 027-84454412/4876 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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