After many years of practice, the author explored the "simplification of innovation in the production of edible fungi." This method can eliminate the expensive equipment, raw materials, drugs, and supplies needed for traditional seed production and save thousands of dollars in investment. The strains produced are particularly resistant to both high and low temperatures. The success rate for inoculation is 100%, and the seed production cycle is shortened by 20 to 30 days. Its simplified method of innovation is:
1. Seed production innovation to simplify the conventional method Potato, glucose, agar, etc. as raw materials, mix and match PDA culture medium, used for planting a first-class test-tube parent species; reform wheat flour, corn, corn The whole nutrient-rich high-nitrogen granular edible fungus kind general culture medium made of grains, corn cobs, tree shoots, etc., optionally made of raw materials, and supplemented with a strain seed coating agent, is used for planting a test tube Female seed, 2 bottled seed, 3 bottled cultivar. The raw material cost of a test tube parent culture medium only costs 3 cents, and the raw material cost of 1 bottle of original species or 1 bottle of cultivated species is only 2 cents, which is both economical and practical.
2. Autoclave innovation to simplify the parent or seed culture medium into a special autoclave, put 1.2 kilograms per square centimeter, and maintain 30 to 40 minutes sterilization under steam pressure; In the “Supor†domestic pressure cooker for rice, when steam is injected from the pressure limiting valve hole, it is kept for 40 to 50 minutes, and it is taken out after cooling to normal pressure.
3. The atmospheric pressure sterilization innovation simplifies the cultivating medium to be sterilized in an atmospheric pressure sterilization pot for 8 to 10 hours; it is reformed to be placed in a space sterilizing bag (a self-made material costs about 30 yuan and can accommodate 500 to 800 bottles). Bacteria 3 to 4 hours.
4. The innovation of inoculation method simplifies the old methods of closed inoculation after supplementing with alcohol lamp flame by fumigation sterilization with formaldehyde, potassium permanganate and other drugs in the inoculation box or inoculation room; innovation is to use “four types†edible fungi Bacteria inoculants, no electricity, no steam, no alcohol, no drugs fumigation sterilization, direct all kinds of edible fungi for the entire process of open inoculation transplantation, the yield of more than 99%.
5. The clinker seed production innovation simplifies the production of grade 3 cultivars of edible fungus such as oyster mushroom, puffball mushroom, red mushroom, coprinus comatus, oyster mushroom, etc., and inoculates and cultivates with “clinker†after sterilization by steam autoclave; For direct raw material inoculation production. After the filling, the middle vent hole was permeabilized, and the whole nutrient-rich nitrogen type granular seed (with a seed amount of 15%) was inserted and cultured at an appropriate temperature.
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