Ultrasonic breaker use tips
- Clicks: 741 Release time: 2011/9/9
First, the ultrasonic pulsator should be inserted into the sample before it can be turned on before use. It can not be operated at no load.
Second, the ultrasonic parameter setting of the ultrasonic breaker: set the key to the instrument working parameters (see the manual for specific settings). For samples that are sensitive to temperature requirements (such as bacteria), the ice bath is generally used outside, and the actual temperature is definitely less than 25 degrees. The protein nucleic acid certainly does not degenerate.
1. Time: The ultrasonic time should not exceed 5 seconds, and the gap time should be greater than or equal to the ultrasonic time to facilitate heat dissipation. The time setting should be based on the principle that the ultrasonic time is short and the number of ultrasound times is large, which can prolong the life of the ultrasonic machine and the probe.
2, ultrasonic power: should not be too large, so as not to splash or foam the sample, such as less than 10ml sample capacity, power should be within 200w, select 2mm ultrasonic probe, and the horn selector switch behind the panel to the corresponding gear; 10- The power of 200ml sample capacity is 200-400w, 6mm ultrasonic probe is used, and the horn behind the panel is driven to the corresponding gear; the capacity of the sample above 200ml is between 300-600w, 10mm ultrasonic probe is used, and the rear of the panel is used. The horn is hit to the corresponding gear; (the small probe power of 2MM is strictly prohibited to exceed 350W)
3. The container of the ultrasonic crusher: How many samples are selected for the beaker, which is advantageous for convection in the ultrasonic to improve the crushing efficiency.
For example, the processing amount of 20ML is good to use a 20ML beaker. For example, 100 ml E. coli sample setting parameters: Ultrasound 5 seconds / gap 5 seconds number 70 times (total time is 10 minutes). Power 300W (for reference only, please consult the technical staff for details) 500ML or so, the power is about 500W-800W.
Third, in order to prevent the ice bath from melting after the ice bath is melted, the sample should be placed in a 1.5ml EP tube and the EP tube should be fixed.
Fourth, the depth of the ultrasonic probe (horn) inserted into the water is about 1.5Cm, the liquid level is above 30mm, the probe should be centered, and it should not be attached. Ultrasonic waves are vertical longitudinal waves. If the insertion is too deep, it is not easy to form convection, which will directly affect the crushing efficiency.
V. Maintenance method: scrub the probe with alcohol after use or use ultrasonic water for ultrasound.
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