The process conditions of ethanol extraction of Xiaoganzhi Granules are preferred. Methods The orthogonal test method was used to determine the extraction rate of curcumin and the yield of extract. The process conditions of ethanol reflux extraction were preferred. Results The optimum conditions for ethanol extraction of Zui were: adding 6 times of 80% ethanol and refluxing for 2 times for 1 h each time. Conclusion This process provides an experimental basis for ensuring the leaching of active ingredients.
Xiaoganzhi Granule is a compound Chinese medicine preparation consisting of Yinchen, Yujin, Alisma, and Atractylodes. It has the functions of clearing away dampness and heat, soothing the liver and nourishing the liver. It has achieved good curative effect in the treatment of obese fatty liver. Yujinzhong curcuminoids have anti-inflammatory, anti-lipid peroxidation, hypoglycemic and hepatoprotective effects [1]; Alisma is an effective part of triterpenoid total aglycones, which has hypolipidemic and anti-fatty liver effects. [2]. In order to better extract the active ingredients of the prescription Chinese herbal medicines and ensure the efficacy of the preparations, the turmeric and Alisma in the other side are extracted with ethanol, and the dregs are decoctioned with other herbs. In this paper, the ethanol extraction process was studied.
1. Instruments and materials 2. METHODS AND RESULTS 2.1 Establishment of process indicators 2.1.1 Determination of curcumin content 4.26 mg of curcumin reference substance dried by phosphorus pentoxide was accurately weighed, dissolved in methanol and made up to 25 ml volumetric flask. Accurately pipette 5 ml of the above solution into a 25 ml volumetric flask, dilute to the mark with methanol, and shake to obtain a reference solution (34.08 μg/ml).
Using high performance liquid chromatography [3], the mobile phase is acetonitrile-4% glacial acetic acid (48:52), the flow rate is 1ml/min, the measurement wavelength is 430nm, and the reference solution is accurately taken, 1, 2, 4, 8, 10, 15 μl, chromatographic analysis according to the above chromatographic conditions, with the injection volume of the reference substance as the abscissa,
The peak area is the ordinate, and the standard curve is drawn. The regression equation is obtained as Y=7.1248x-0.0513, and the correlation coefficient is r=0.9998. The results showed that the linear relationship of curcumin in the range of 0.03408～0.5112μg was good, and the stability, precision and recovery rate of the method were verified by the method, and the curcumin content was determined.
2.2 Selection of ethanol extraction times 2.3 Ethanol reflux extraction process optimization 2.3.1 Orthogonal test design 2.3.2 Operation method and results 2.3.3 Variance analysis and conclusion 2.3.4zui good process verification 3. Discussion The ethanol extraction process has not appeared in the orthogonal test. Therefore, the amplification test was carried out for 3 times. The average curcumin content in the extract was 1.284 mg/g crude drug, and the average extraction rate was 84.12%, slightly higher than The good value of zui in the orthogonal test, while the dry paste yield is within 15%, in line with the granulation molding requirements, indicating that the Zuijia process conditions are feasible, providing an experimental basis for ensuring the leaching of the active ingredients. Other medicinal materials in the prescription, the effective parts are water-soluble and parts such as capillaris and atractylodes are volatile oils, so they will be extracted by water decoction and the volatile oil will be collected at the same time.
Xiaoganzhi Granule is a compound Chinese medicine preparation consisting of Yinchen, Yujin, Alisma, and Atractylodes. It has the functions of clearing away dampness and heat, soothing the liver and nourishing the liver. It has achieved good curative effect in the treatment of obese fatty liver. Yujinzhong curcuminoids have anti-inflammatory, anti-lipid peroxidation, hypoglycemic and hepatoprotective effects [1]; Alisma is an effective part of triterpenoid total aglycones, which has hypolipidemic and anti-fatty liver effects. [2]. In order to better extract the active ingredients of the prescription Chinese herbal medicines and ensure the efficacy of the preparations, the turmeric and Alisma in the other side are extracted with ethanol, and the dregs are decoctioned with other herbs. In this paper, the ethanol extraction process was studied.
1. Instruments and materials 2. METHODS AND RESULTS 2.1 Establishment of process indicators 2.1.1 Determination of curcumin content 4.26 mg of curcumin reference substance dried by phosphorus pentoxide was accurately weighed, dissolved in methanol and made up to 25 ml volumetric flask. Accurately pipette 5 ml of the above solution into a 25 ml volumetric flask, dilute to the mark with methanol, and shake to obtain a reference solution (34.08 μg/ml).
Using high performance liquid chromatography [3], the mobile phase is acetonitrile-4% glacial acetic acid (48:52), the flow rate is 1ml/min, the measurement wavelength is 430nm, and the reference solution is accurately taken, 1, 2, 4, 8, 10, 15 μl, chromatographic analysis according to the above chromatographic conditions, with the injection volume of the reference substance as the abscissa,
The peak area is the ordinate, and the standard curve is drawn. The regression equation is obtained as Y=7.1248x-0.0513, and the correlation coefficient is r=0.9998. The results showed that the linear relationship of curcumin in the range of 0.03408～0.5112μg was good, and the stability, precision and recovery rate of the method were verified by the method, and the curcumin content was determined.
2.2 Selection of ethanol extraction times 2.3 Ethanol reflux extraction process optimization 2.3.1 Orthogonal test design 2.3.2 Operation method and results 2.3.3 Variance analysis and conclusion 2.3.4zui good process verification 3. Discussion The ethanol extraction process has not appeared in the orthogonal test. Therefore, the amplification test was carried out for 3 times. The average curcumin content in the extract was 1.284 mg/g crude drug, and the average extraction rate was 84.12%, slightly higher than The good value of zui in the orthogonal test, while the dry paste yield is within 15%, in line with the granulation molding requirements, indicating that the Zuijia process conditions are feasible, providing an experimental basis for ensuring the leaching of the active ingredients. Other medicinal materials in the prescription, the effective parts are water-soluble and parts such as capillaris and atractylodes are volatile oils, so they will be extracted by water decoction and the volatile oil will be collected at the same time.
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